抗 AGEs モノクローナル抗体 (Clone No.6D12)


容量	10μg/40μL
価格(税別)	お問い合わせください
Data Sheet

免疫抗原：	AGEs-BSA
GenBank ID：	–
免疫動物：	mouse（マウス）
精製方法：	ProteinG
抗体サブクラス：	IgG1

適用	(0.1-0.5μg/mL) (0.25-5μg/mL) (2μg/mL)
交差性
その他	AGEs(Advanced Glycation End Products)は、タンパク質の非酵素的糖付加反応（メイラード反応）により、シッフ塩基、アマドリ転移生成物（前期生成物）を経由し、脱水、酸化、縮合などの複雑な反応を受けて形成される最終生成物です。AGEs は、蛍光・褐色・分子架橋形成などの特徴の他、AGEs 受容体により認識されるという生化学的特性を有しています。近年の抗 AGEs 抗体による解析の結果、（１)ヒト水晶体（加齢に伴う増加）、（２）糖尿病性腎症や慢性腎不全患者の腎近位尿細管、（３）糖尿病患者の網膜、（４）糖尿病性神経障害患者の末梢神経、（５）粥状動脈硬化病変部、（６）透析性アミロイド－シスのβ2-マイクログロブリン、（７）アルツハイマー病患者の老人斑、（８）CAPD 患者の腹膜、（９）弾力線維症の皮膚のエラスチン、（１０）セロイド／リポフスチン沈着部位などにAGEsが蓄積することが分かってきました。これらの知見は、老化自体や老化に伴う慢性疾患にAGEs が深く関与していることを示唆しています。本抗体(6D12)は、加齢に伴う慢性疾患の研究に非常に有用であると思われます。
文献	J Biol Chem. 1991 Apr 25;266(12):7329-32. Immunochemical approach to characterize advanced glycation end products of the Maillard reaction. Evidence for the presence of a common structure. Horiuchi S, Araki N, Morino Y. Am J Pathol. 1995 Sep;147(3):654-67. Immunohistochemical and ultrastructural detection of advanced glycation end products in atherosclerotic lesions of human aorta with a novel specific monoclonal antibody. Kume S, Takeya M, Mori T, Araki N, Suzuki H, Horiuchi S, Kodama T, Miyauchi Y, Takahashi K. Biochemistry. 1996 Jun 18;35(24):8075-83. N (epsilon)-(carboxymethyl)lysine protein adduct is a major immunological epitope in proteins modified with advanced glycation end products of the Maillard reaction. Ikeda K, Higashi T, Sano H, Jinnouchi Y, Yoshida M, Araki T, Ueda S, Horiuchi S. J Invest Dermatol. 1997 May;108(5):797-802. Photo-enhanced modification of human skin elastin in actinic elastosis by N(epsilon)-(carboxymethyl)lysine, one of the glycoxidation products of the Maillard reaction. Mizutari K, Ono T, Ikeda K, Kayashima K, Horiuchi S. J Clin Pathol. 1998 Jul;51(7):515-9. Immunohistochemical localisation of advanced glycation end products in pulmonary fibrosis. Matsuse T, Ohga E, Teramoto S, Fukayama M, Nagai R, Horiuchi S, Ouchi Y. Nephrol Dial Transplant. 1999 Jun;14(6):1541-9. Accumulation of advanced glycation end products in the peritoneal vasculature of continuous ambulatory peritoneal dialysis patients with low ultra-filtration. Honda K, Nitta K, Horita S, Yumura W, Nihei H, Nagai R, Ikeda K, Horiuchi S. Diabetes. 1999 Sep;48(9):1842-9. Autoantibody against N(epsilon)-(carboxymethyl)lysine: an advanced glycation end product of the Maillard reaction. Shibayama R, Araki N, Nagai R, Horiuchi S. Invest Ophthalmol Vis Sci. 2000 Feb;41(2):362-8. Advanced glycation end products in diabetic corneas. Kaji Y, Usui T, Oshika T, Matsubara M, Yamashita H, Araie M, Murata T, Ishibashi T, Nagai R, Horiuchi S, Amano S. Brain Dev. 2000 Jun;22(4):234-8. Unique astrocytic inclusion in a 2 month-old baby showing Leigh-like brain lesions with lactic acidosis. Yamamoto T, Armstrong D, Shibata N, Kato Y, Kobayashi M. Lab Invest. 2001 Jun;81(6):845-61. Immunohistochemical distribution and quantitative biochemical detection of advanced glycation end products in fetal to adult rats and in rats with streptozotocin-induced diabetes. Ling X, Nagai R, Sakashita N, Takeya M, Horiuchi S, Takahashi K. Am J Physiol Heart Circ Physiol. 2002 Oct;283(4):H1387-97. Role of ANG II in coronary capillary angiogenesis at the insulin-resistant stage of a NIDDM rat model. Jesmin S, Hattori Y, Sakuma I, Mowa CN, Kitabatake A. Am J Physiol Heart Circ Physiol. 2002 Oct;283(4):H1387-97. Role of ANG II in coronary capillary angiogenesis at the insulin-resistant stage of a NIDDM rat model. Jesmin S, Hattori Y, Sakuma I, Mowa CN, Kitabatake A. J Biol Chem. 2002 Dec 13;277(50):48905-12 Identification in human atherosclerotic lesions of GA-pyridine, a novel structure derived from glycolaldehyde-modified proteins. Nagai R, Hayashi CM, Xia L, Takeya M, Horiuchi S. Perit Dial Int. 2003 Jan-Feb;23(1):23-32. Chemical and immunological characterization of oxidative nonenzymatic protein modifications in dialysis fluids. Ruiz MC, Portero-Otín M, Pamplona R, Requena JR, Prat J, Lafarga MA, Borràs M, Bellmunt MJ. Biol Pharm Bull. 2004 Oct;27(10):1565-71. Overproduction of N(epsilon)-(carboxymethyl)lysine-induced neovascularization in cultured choroidal explant of streptozotocin-diabetic rat. Kobayashi S, Suzuki M, Tsuneki H, Nagai R, Horiuchi S, Hagino N. Exp Eye Res. 2005 Apr;80(4):567-80. Mechanisms for the induction of HNE- MDA- and AGE-adducts, RAGE and VEGF in retinal pigment epithelial cells. Zhou J, Cai B, Jang YP, Pachydaki S, Schmidt AM, Sparrow JR. J Biol Chem. 2005 Jun 3;280(22):21522-30. Proteins in human brain cortex are modified by oxidation, glycoxidation, and lipoxidation. Effects of Alzheimer disease and identification of lipoxidation targets. Pamplona R, Dalfó E, Ayala V, Bellmunt MJ, Prat J, Ferrer I, Portero-Otín M. Lab Invest. 2006 Apr;86(4):357-68. Expression of Smad1 is directly associated with mesangial matrix expansion in rat diabetic nephropathy. Matsubara T, Abe H, Arai H, Nagai K, Mima A, Kanamori H, Sumi E, Takahashi T, Matsuura M, Iehara N, Fukatsu A, Kita T, Doi T. Proc Natl Acad Sci U S A. 2006 May 16;103(20):7700-5. Elimination of damaged proteins during differentiation of embryonic stem cells Hernebring M, Brolén G, Aguilaniu H, Semb H, Nyström T. Intern Med. 2006;45(7):435-41. Relationship between the expression of advanced glycation end-products (AGE) and the receptor for AGE (RAGE) mRNA in diabetic nephropathy. Suzuki D, Toyoda M, Yamamoto N, Miyauchi M, Katoh M, Kimura M, Maruyama M, Honma M, Umezono T, Yagame M. J Neurochem. 2006 Oct;99(1):177-85. Glial fibrillary acidic protein is a major target of glycoxidative and lipoxidative damage in Pick’s disease. Muntané G, Dalfó E, Martínez A, Rey MJ, Avila J, Pérez M, Portero M, Pamplona R, Ayala V, Ferrer I. J Biomol Tech. 2006 Sep;17(4):283-92. Optimization of the Tet-On system for inducible expression of RAGE. Shaikh S, Nicholson LF. Br J Pharmacol. 2006 April; 147(8): 944–950. Aminoguanidine prevents arterial stiffening and cardiac hypertrophy in streptozotocin-induced diabetes in rats Kuo-Chu Chang, Kwan-Lih Hsu, Chuen-Den Tseng, Yue-Der Lin, Yi-Li Cho, and Yung-Zu Tseng J Biomol Tech. 2006 September; 17(4): 283–292. Optimization of the Tet-On System for Inducible Expression of RAGE Shamim Shaikh and Louise F.B. Nicholson J Biol Chem. 2007 Mar 9;282(10):6984-91. Effect of pseudophosphorylation and cross-linking by lipid peroxidation and advanced glycation end product precursors on tau aggregation and filament formation. Kuhla B, Haase C, Flach K, Lüth HJ, Arendt T, Münch G Diabetes. 2007 Feb;56(2):363-72. Macrophage scavenger receptor-a-deficient mice are resistant against diabetic nephropathy through amelioration of microinflammation. Usui HK, Shikata K, Sasaki M, Okada S, Matsuda M, Shikata Y, Ogawa D, Kido Y, Nagase R, Yozai K, Ohga S, Tone A, Wada J, Takeya M, Horiuchi S, Kodama T, Makino H. J Biol Chem. 2007 Aug 10;282(32):23427-36. Vimentin is the specific target in skin glycation. Structural prerequisites, functional consequences, and role in skin aging. Kueper T, Grune T, Prahl S, Lenz H, Welge V, Biernoth T, Vogt Y, Muhr GM, Gaemlich A, Jung T, Boemke G, Elsässer HP, Wittern KP, Wenck H, Stäb F, Blatt T. J Gerontol A Biol Sci Med Sci. 2007 Nov;62(11):1204-10. Advanced glycation end-product accumulation and associated protein modification in type II skeletal muscle with aging. Snow LM, Fugere NA, Thompson LV. J Neurosci Res. 2008 Jul;86(9):2071-82. Advanced glycation end products induce in vitro cross-linking of alpha-synuclein and accelerate the process of intracellular inclusion body formation. Shaikh S, Nicholson LF. Ann N Y Acad Sci. 2008 Apr;1126:328-32. Modification of vimentin: a general mechanism of nonenzymatic glycation in human skin. Kueper T, Grune T, Muhr GM, Lenz H, Wittern KP, Wenck H, Stäb F, Blatt T. Biogerontology. 2009 Jun;10(3):299-309. Identification of Hsc70 as target for AGE modification in senescent human fibroblasts. Unterluggauer H, Micutkova L, Lindner H, Sarg B, Hernebring M, Nystrom T, Jansen-Dürr P. Spine (Phila Pa 1976). 2009 Jul 1;34(15):1544-8. Up-regulation in receptor for advanced glycation end-products in inflammatory circumstances in bovine coccygeal intervertebral disc specimens in vitro. Yoshida T, Park JS, Yokosuka K, Jimbo K, Yamada K, Sato K, Takeuchi M, Yamagishi S, Nagata K. Pathobiology. 2009;76(5):227-34. Influence of insulin and muscle fiber type in nepsilon-(carboxymethyl)-lysine accumulation in soleus muscle of rats with streptozotocin-induced diabetes mellitus. Snow LM, Thompson LV. Lab Invest. 2010 Aug;90(8):1189-98. Oxidative stress-associated rise of hepatic protein glycation increases inflammatory liver injury in uncoupling protein-2 deficient mice. Kuhla A, Hettwer C, Menger MD, Vollmar B. Am J Physiol Renal Physiol. 2010 Dec;299(6):F1451-61. Eicosapentaenoic acid restores diabetic tubular injury through regulating oxidative stress and mitochondrial apoptosis. Taneda S, Honda K, Tomidokoro K, Uto K, Nitta K, Oda H. PLoS One. 2010; 5(2): e9052. Ribosylation Rapidly Induces α-Synuclein to Form Highly Cytotoxic Molten Globules of Advanced Glycation End Products Lan Chen, Yan Wei, Xueqing Wang, and Rongqiao He J Hepatol. 2011 Jan 11. Increasing Hepatic Arteriole Wall Thickness and Decreased Luminal Diameter Occur With Increasing Age in Normal Livers. Fiel MI, Deniz K, Elmali F, Schiano TD. J Immunol. 2011 Mar 1;186(5):3248-57. Septic shock is associated with receptor for advanced glycation end products ligation of LPS. Yamamoto Y, Harashima A, Saito H, Tsuneyama K, Munesue S, Motoyoshi S, Han D, Watanabe T, Asano M, Takasawa S, Okamoto H, Shimura S, Karasawa T, Yonekura H, Yamamoto H. Phytother Res. 2011 Feb 10. doi: 10.1002/ptr.3423 Effect of Cinnamoyl and Flavonol Glucosides Derived from Cherry Blossom Flowers on the Production of Advanced Glycation End Products (AGEs) and AGE-induced Fibroblast Apoptosis. Shimoda H, Nakamura S, Morioka M, Tanaka J, Matsuda H, Yoshikawa M. Evid Based Complement Alternat Med. 2011;2011:784136. Combination of Medicinal Herbs KIOM-79 Reduces Advanced Glycation End Product Accumulation and the Expression of Inflammatory Factors in the Aorta of Zucker Diabetic Fatty Rats. Sohn E, Kim J, Jeong IH, Kim CS, Kim YS, Kim JS. J Cereb Blood Flow Metab. 2011 Apr 20. Cilostazol minimizes venous ischemic injury in diabetic and normal rats. Wajima D, Nakamura M, Horiuchi K, Takeshima Y, Nishimura F, Nakase H. Brain Res. 2011 May 4;1388:32-8 Atorvastatin and pitavastatin protect cerebellar Purkinje cells in AD model mice and preserve the cytokines MCP-1 and TNF-α. Kozuki M, Kurata T, Miyazaki K, Morimoto N, Ohta Y, Ikeda Y, Abe K.
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