抗ヒト PEDF モノクローナル抗体 (Clone No.14K)


容量	50μg/200μL
価格(税別)	お問い合わせください
Data Sheet

免疫抗原：	部分ペプチド
GenBank ID：	5176
免疫動物：	mouse（マウス）
精製方法：	ProteinG
抗体サブクラス：	IgG1

適用	(0.5-1.0μg/mL)
交差性
その他	色素上皮由来因子（pigment epithelium-derived factor:PEDF ）は、網膜に隣接する神経の成長、分化及び生存に有益な神経親和性因子です。網膜だけでなく、中枢神経にPEDF のmRNA が広範囲に分布しており、神経に対して保護的な効果を持つことが示唆されています。また、PEDF にはScience(285:245-248,1999)に、最強の血管新生抑制作用があることが報告されており、海外では、眼科分野の遺伝子治療の研究にも用いられて注目されています。本抗体は、網膜色素上皮細胞(RPE)培養上清中のPEDF をWestern blotting 法にて検出できます。血管新生、神経系の研究にご使用ください。
文献	Biochem Biophys Res Commun. 2002 Aug 30;296(4):877-82. Pigment epithelium-derived factor protects cultured retinal pericytes from advanced glycation end product-induced injury through its antioxidative properties. Yamagishi S, Inagaki Y, Amano S, Okamoto T, Takeuchi M, Makita Z. Invest Ophthalmol Vis Sci. 2003 Dec;44(12):5370-5. Reduced retinal angiogenesis in MMP-2-deficient mice. Ohno-Matsui K, Uetama T, Yoshida T, Hayano M, Itoh T, Morita I, Mochizuki M. Cell Tissue Res. 2005 Jun;320(3):437-45. Pigment epithelium-derived factor (PEDF) blocks angiotensin II signaling in endothelial cells via suppression of NADPH oxidase: a novel anti-oxidative mechanism of PEDF. Yamagishi S, Nakamura K, Ueda S, Kato S, Imaizumi T. J Clin Endocrinol Metab. 2006 Jun;91(6):2447-50. Elevated serum levels of pigment epithelium-derived factor in the metabolic syndrome. Yamagishi S, Adachi H, Abe A, Yashiro T, Enomoto M, Furuki K, Hino A, Jinnouchi Y, Takenaka K, Matsui T, Nakamura K, Imaizumi T. Microvasc Res. 2006 May;71(3):222-6. Pigment-epithelium-derived factor (PEDF) inhibits angiotensin-II-induced vascular endothelial growth factor (VEGF) expression in MOLT-3 T cells through anti-oxidative properties. Yamagishi S, Matsui T, Nakamura K, Yoshida T, Shimizu K, Takegami Y, Shimizu T, Inoue H, Imaizumi T. FEBS Lett. 2006 May 15;580(11):2788-96. Pigment epithelium-derived factor (PEDF) inhibits advanced glycation end product (AGE)-induced C-reactive protein expression in hepatoma cells by suppressing Rac-1 activation. Yoshida T, Yamagishi S, Nakamura K, Matsui T, Imaizumi T, Takeuchi M, Ueno T, Sata M. J Biol Chem. 2006 Jul 21;281(29):20213-20. Pigment epithelium-derived factor inhibits advanced glycation end product-induced retinal vascular hyperpermeability by blocking reactive oxygen species-mediated vascular endothelial growth factor expression. Yamagishi S, Nakamura K, Matsui T, Inagaki Y, Takenaka K, Jinnouchi Y, Yoshida Y, Matsuura T, Narama I, Motomiya Y, Takeuchi M, Inoue H, Yoshimura A, Bucala R, Imaizumi T. Microvasc Res. 2006 Jul-Sep;72(1-2):86-90. Pigment epithelium-derived factor (PEDF) prevents diabetes- or advanced glycation end products (AGE)-elicited retinal leukostasis. Yamagishi S, Matsui T, Nakamura K, Takeuchi M, Imaizumi T. Life Sci. 2006 Oct 19;79(21):1981-7. Pigment epithelium-derived factor (PEDF) blocks the interleukin-6 signaling to C-reactive protein expression in Hep3B cells by suppressing Rac-1 activation. Yoshida T, Yamagishi S, Nakamura K, Matsui T, Imaizumi T, Inoue H, Ueno T, Sata M. Horm Metab Res. 2006 Aug;38(8):546-8. Pigment epithelium-derived factor (PEDF) blocks angiotensin II-induced T cell adhesion to endothelial cells by suppressing intercellular adhesion molecule-1. Yamagishi SI, Kikuchi S, Nakamura K, Matsui T, Makino T, Norisugi O, Shimizu T, Inoue H, Imaizumi T. Med Chem. 2006 May;2(3):265-9. Pigment epithelium-derived factor (PEDF) blocks angiotensin II-induced T cell proliferation by suppressing autocrine production of interleukin-2. Yamagishi S, Kikuchi S, Nakamura K, Matsui T, Takeuchi M, Inoue H. Ophthalmic Res. 2007;39(2):92-7. Pigment-epithelium-derived factor suppresses expression of receptor for advanced glycation end products in the eye of diabetic rats. Yamagishi S, Matsui T, Nakamura K, Yoshida T, Takeuchi M, Inoue H, Yoshida Y, Imaizumi T. Horm Metab Res. 2007 Mar;39(3):233-5. Pigment epithelium-derived factor (PEDF) blocks advanced glycation end product (AGE)-induced angiogenesis in vitro. Yamagishi SI, Nakamura K, Matsui T, Yoshida T, Takeuchi M, Imaizumi T. Am J Pathol. 2007 Jun;170(6):2159-70. Pigment epithelium-derived factor inhibits neointimal hyperplasia after vascular injury by blocking NADPH oxidase-mediated reactive oxygen species generation. Nakamura K, Yamagishi S, Matsui T, Yoshida T, Takenaka K, Jinnouchi Y, Yoshida Y, Ueda S, Adachi H, Imaizumi T. Protein Pept Lett. 2007;14(6):615-7. Pigment epithelium-derived factor (PEDF) inhibits angiotensin II-induced smooth muscle cell proliferation through its anti-oxidative properties. Yamagishi S, Matsui T, Nakamura K, Imaizumi T. Protein Pept Lett. 2007;14(8):832-5. Pigment epithelium-derived factor (PEDF) prevents advanced glycation end products (AGEs)-elicited endothelial nitric oxide synthase (eNOS) reduction through its anti-oxidative properties. Yamagishi S, Ueda S, Matsui T, Nakamura K, Imaizumi T, Takeuchi M, Okuda S. J Int Med Res. 2007 Nov-Dec;35(6):896-9. Pigment epithelium-derived factor inhibits vascular endothelial growth factor-induced vascular hyperpermeability both in vitro and in vivo. Yamagishi S, Abe R, Jinnouchi Y, Matsui T, Imaizumi T, Inoue H. Horm Metab Res. 2008 Sep;40(9):620-5. Pigment epithelium-derived factor (PEDF) ameliorates advanced glycation end product (AGE)-induced hepatic insulin resistance in vitro by suppressing Rac-1 activation. Yoshida T, Yamagishi S, Nakamura K, Matsui T, Imaizumi T, Takeuchi M, Koga H, Ueno T, Sata M. Clin Exp Med. 2009 Mar;9(1):73-6. Administration of pigment epithelium-derived factor prolongs bleeding time by suppressing plasminogen activator inhibitor-1 activity and platelet aggregation in rats. Yamagishi S, Matsui T, Nakamura K, Takenaka K. Nephrol Dial Transplant. 2009 May;24(5):1397-406. Administration of pigment epithelium-derived factor (PEDF) reduces proteinuria by suppressing decreased nephrin and increased VEGF expression in the glomeruli of adriamycin-injected rats. Fujimura T, Yamagishi S, Ueda S, Fukami K, Shibata R, Matsumoto Y, Kaida Y, Hayashida A, Koike K, Matsui T, Nakamura K, Okuda S. Diabetes Metab Res Rev. 2009 Mar;25(3):266-71. Pigment epithelium-derived factor (PEDF) prevents platelet activation and aggregation in diabetic rats by blocking deleterious effects of advanced glycation end products (AGEs). Yamagishi S, Matsui T, Takenaka K, Nakamura K, Takeuchi M, Inoue H. Diabetes Metab Res Rev. 2009 Oct;25(7):678-86. Protective role of pigment epithelium-derived factor (PEDF) in early phase of experimental diabetic retinopathy. Yoshida Y, Yamagishi S, Matsui T, Jinnouchi Y, Fukami K, Imaizumi T, Yamakawa R. Int J Cardiol. 2010 Oct 8;144(2):283-5. Pigment epithelium-derived factor (PEDF) inhibits diabetes- or advanced glycation end product (AGE)-induced platelet CD40 ligand overexpression in rats. Yamagishi S, Matsui T, Ueda S, Takeuchi M. Am J Pathol. 2010 Jan;176(1):168-76. Pigment epithelium-derived factor inhibits lysosomal degradation of Bcl-xL and apoptosis in HepG2 cells. Kawaguchi T, Yamagishi S, Itou M, Okuda K, Sumie S, Kuromatsu R, Sakata M, Abe M, Taniguchi E, Koga H, Harada M, Ueno T, Sata M. J Dermatol Sci. 2010 Mar;57(3):183-91. Topical application of anti-angiogenic peptides based on pigment epithelium-derived factor can improve psoriasis. Abe R, Yamagishi S, Fujita Y, Hoshina D, Sasaki M, Nakamura K, Matsui T, Shimizu T, Bucala R, Shimizu H. Curr Mol Med. 2010 Apr;10(3):317-20. Development of enzyme-linked immunosorbent assay system for PEDF and its clinical utility. Fukami K, Yamagishi SI, Okuda S. Microvasc Res. 2010 Sep;80(2):227-32. Pigment epithelium-derived factor inhibits advanced glycation end product-elicited mesangial cell damage by blocking NF-kappaB activation. Ide Y, Matsui T, Ishibashi Y, Takeuchi M, Yamagishi S.
共同研究	東京医科歯科大学森田育男先生、大野京子先生