論文・学会発表

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糖鎖解析 論文発表

1. “Glycoblotting method allows for rapid and efficient glycome profiling of human Alzheimer’s disease brain, serum and cerebrospinal fluid towards potential biomarker discovery.”
   Gizaw S., Ohashi T., Tanaka M., Hinou H., Nishimura S-I., BBA-GenSubjects 1860, 1716-1727 (2016)
   PMID: 26968461
2. “Increase of Sialylated N-Glycansin Eyes with Neovascular Glaucoma Secondary to Proliferative DiabeticRetinopathy.”
   Inafuku, S., Noda, K., Amano, M., Nishimura, S. –I., Ishida, S. Curr. Eye Res. 41, 721-724 (2016)
   PMID: 26177396
3. “Alteration of serum N-glycan profile in patients with autoimmune pancreatitis” 
   Tomoda T., Nouso K., Kato H., Miyahara K., Dohi C., Morimoto Y., Kinugasa H., Akimoto Y., Matsumoto K., Yamamoto N., Noma Y., Horiguchi S., Tsutsumi K., Amano M., Nishimura S-I., Yamamoto K., Pancreatology 16, 44-51 (2015)
   PMID: 26052067
4. “Serum N-glycan profiles in patients with intraductal papillary mucinous neoplasms of the pancreas”
   Akimoto Y., Nouso K., Kato H., Miyahara K., Dohi C., Morimoto Y., Kinugasa H., Tomoda T., Yamamoto N., Tsutsumi K., Kuwaki K., Onishi H., Ikeda F., Nakamura S., Shiraha H.,  Takaki A., Okada H., Amano M., Nishimura S-I., Yamamoto K.,  Pancreatology 15, 432-438 (2015)
   PMID: 26177396
5. “Large-scale Glycomics of Livestock: Discovery of Highly Sensitive Serum Biomarkers Indicating an Environmental Stress Affecting Immune Responses and Productivity of Holstein Dairy Cows”
   Rehan I., Ueda K., Mitani T., Amano M., Hinou H, Ohashi T., Kondo S., and Nishimura S-I.,  J. Agric. Food. Chem. 63, 10578-10590 (2015).
   PMID: 26595672
6. “Alteration of N-Glycan Profiles in Diabetic Retinopathy.”
   Inafuku, S., Noda, K., Amano, M., Ohashi, T., Yoshizawa, C., Saito, W., Murata, M., Kanda, A., Nishimura, S. –I., Ishida, S. Invest. Ophthalmol. Vis. Sci. 56, 5316-5322 (2015).
   PMID: 26258617
7. “A comprehensive glycome profiling of Huntington’s disease transgenic mice.”
   Gizaw, S., Koda, T., Amano, M., Kamimura, K., Ohashi, T., Hinou, H., Nishimura, S. –I. Biochim. Biophys. Acta. 1850, 1704-1718 (2015).
   PMID: 25907331
8. “Prognostic Value of Altered N-Glycosylation of Circulating Glycoproteins in Patients With Unresectable Pancreatic Cancer Treated With Gemcitabine”
   Miyahara K., Nouso K., Morimoto Y., Kinugasa H., Kato H., Yamamoto N., Tsutsumi K., Kuwaki., K., Onishi H., Ikeda F., Nakamura S., Shiraha H., Takaki A., Nakahara T., Miura Y., Asada H., Amano M., Nishimura S-I., Yamamoto K., Pancreas 44, 551-556 (2015)
   PMID: 25875794
9. “Alteration of N-glycan profiles in patients with chronic hepatitis and hepatocellular carcinoma.”
   Miyahara, K., Nouso, K., Dohi, C., Morimoto, Y., Kinugasa, H., Wada, N., Takeuchi, Y., Kuwaki, K., Onishi, H., Ikeda, F., Miyake, Y., Nakamura, S., Shiraha, H., Takaki, A., Amano, M., Nishimura, S. –I., Yamamoto, K. Hepatol. Res. 45. 986-993 (2015).
   PMID: 25495282
10. “Use of non-invasive serum glycan markers to distinguish non-alcoholic steatohepatitis from simple steatosis.”
    Yamasaki, Y., Nouso, K., Miyahara, K., Wada, N., Dohi, C., Morimoto, Y., Takeuchi, Y., Yasunaka, T., Kuwaki, K., Onishi, H., Ikeda, F., Miyake, Y., Nakamura, S., Shiraha, H., Takaki, A., Amano, M., Nishimura, S. –I., Yamamoto, K. J. Gastroen. Hepatol. 30, 528-534 (2015).
    PMID: 25168691
11. “Application of glyco-blotting for identification of structures of polysaccharides causing membrane fouling in a pilot-scale membrane bioreactor treating municipal wastewater.”
    Kimura, K., Nishimura, S. –I., Miyoshi, R., Hoque, A., Miyoshi, T., Watanabe, Y. Bioresource Technol. 179, 180-186 (2015).
    PMID: 25544495
12. “Serum tri- and tetra-antennary N-glycan is a potential predictive biomarker for castration-resistant prostate cancer.”
    Ishibashi, Y., Tobisawa, Y., Hatakeyama, S., Ohashi, T., Tanaka, M., Narita, S., Koie, T., Habuchi, T., Nishimura, S. –I., Ohyama, C. The Prostate 74, 1521-1529 (2014).
    PMID: 25154914
13. “A comparison of N-glycan profiles in human plasma and vitreous fluid.”
    Inafuku, S., Noda, K., Amano, M., Ohashi, T., Yoshizawa, C., Saito, W., Kanda, A., Nishimura, S. –I., Ishida, S. Graefe’s Arch. Clin. Exp. Ophthalmol. 252, 1235-1243 (2014).
    PMID: 24912590
14. “Discovery of novel differentiation markers in the early stage of chondrogenesis by glycoform-focused reverse proteomics and genomics.”
    Ishihara, T., Kakiya, K., Takahashi, K., Miwa, H., Rokushima, M., Yoshinaga, T., Tanaka, Y., Ito, T., Togame, H., Takemoto, H., Amano, M., Iwasaki, N., Minami, A., Nishimura, S. –I. Biochim. Biophys. Acta. 1840, 645-655 (2014).
    PMID: 24161698
15. “Serum glycan as a prognostic marker in patients with advanced hepatocellular carcinoma treated with sorafenib.”
    Miyahara, K., Nouso, K., Miyake, Y., Nakamura, S., Obi, S., Amano, M., Hirose, K., Nishimura, S. –I., Yamamoto, K. Hepatology 59, 355-356 (2014).
    PMID: 23729393
16. “Serum glycan markers for evaluation of disease activity and prediction of clinical course in patients with ulcerative colitis.”
    Miyahara, K., Nouso, K., Saito, S., Hiraoka, S., Harada, K., Takahashi, S., Morimoto, Y., Kobayashi, S., Ikeda, F., Miyake, Y., Shiraha, H., Takaki, A., Amano, M., Nishimura, S. –I., Yamamoto, K. PLOS ONE 8, e74861 (2013).
    PMID: 24116015
17. “Clinical utility of high-throughput glycome analysis in patients with pancreatic cancer.”
    Nouso, K., Amano, M., Ito, M. Y., Miyahara, K., Morimoto, Y., Kato, H., Tsutsumi, K., Tomoda, T., Yamamoto, N., Nakamura, S., Kobayashi, S., Kuwaki, K., Hagihara, H., Onishi, H., Miyake, Y., Ikeda, F., Shiraha, H., Takaki, A., Nakahara, T., Nishimura, S. –I., Yamamoto, K. J. Gastroenterology. 48, 1171-1179 (2013).
    PMID: 23307043
18. “Effect of ganglioside GM3 synthase gene knockout on the glycoprotein N-glycan profile of mouse embryonic fibroblast.”
    Nagahori, N., Yamashita, T., Amano, M., Nishimura, S. –I. ChemBioChem 14, 73-82 (2013).
    PMID: 23225753
19. “Glycoblotting-based high throughput protocol for the structural characterization of hyaluronan degradation products during enzymatic fragmentation.”
    Furukawa, T., Arai, M., Garcia-Martin, F., Amano, M., Hinou, H., Nishimura, S. –I. Glycoconjugate J. 30, 171-182 (2013).
    PMID: 22638863
20. “Further examination of polysaccharides causing membrane fouling in membrane bioreactors (MBRs): Application of lectin affinity chromatography and MALDI-TOF/MS.”
    Kimura, K., Tanaka, I., Nishimura, S. –I., Miyoshi, R., Miyoshi, T., Watanabe, Y. Water Res. 46, 5725-5734 (2012).
    PMID: 22921396
21. “Effects of single genetic damage in carbohydrate-recognizing proteins in mouse serum N-glycan profile revealed by simple glycotyping analysis.”
    Amano, M., Hashimoto, R., Nishimura, S. –I. ChemBioChem. 13, 451-464 (2012).
    PMID: 22271523

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糖鎖合成 論文発表

1. “Novel β-galactosynthase – β-mannosynthase dual activity of β-galactosidase from Aspergillus oryzae uncovered using monomer sugar substrates”
   Roger S. Tan, Hinou H., and Nishimura S-I., RSC Adv. 6, 50833-50836 (2016)
2. “Synthetic Human NOTCH1 EGF Modules Unraveled Molecular Mechanisms for the Structural and Functional Roles of Calcium Ions and O-glycans in the Ligand-binding Region”
   Hayakawa S., Koide R., Hinou H., Nishimura S-I., Biochemistry 55, 776-787 (2016)
   PMID: 26765751
3. “Solid-phase Synthesis of C-mannosylated Glycopeptide on WSXWS Motif of Human Erythropoietin Receptor”
   Hinou H., Abe Y., Hayakawa H., Naruchi K., Fjitani N., Nishimura S-I., Tetrahedron let. 57, 791-795 (2016)
4. “Rapid Endolysosomal Escape and Controlled Intracellular Trafficking of Cell Surface Mimetic Quantum-Dots-Anchored Peptides and Glycopeptides.”
   Roger, S. Tan., Naruchi, K., Amano, M., Hinou, H., Nishimura, S. –I. ACS Chem. Biol. 10, 2073-2086 (2015).
   PMID: 26107406
5. “Synthesis of neoglycosphingolipid from methoxyamino-functionalized ceramide.”
   Ishida, J., Hinou, H., Naruchi, K., Nishimura, S. –I. Bioorg. Med. Chem. Lett. 24, 1197-1200 (2014).
   PMID: 24461288
6. “A straightforward protocol for the preparation of high performance microarray displaying synthetic MUC1 glycopeptides.”
   Matsushita, T., Takada, W., Igarashi, K., Naruchi, K., Miyoshi, R., Garcia-Martin, F., Amano, M., Hinou, H., Nishimura, S. –I. Biochim. Biophys. Acta. 1840, 1105-1116 (2014).
   PMID: 24246952
7. “A new approach for the synthesis of hyperbranched N-glycan core structures from locust bean gum.”
   Kumar, R., Naruchi, K., Miyoshi, R., Hinou, H., Nishimura, S. –I. Org. Lett. 15, 6278-6281 (2013).
   PMID: 24261553
8. “Attomolar detection of influenza A virus hemagglutinin human H1 and avian H5 using glycan-blotted field effect transistor biosensor.”
   Hideshima, S., Hinou, H., Ebihara, D., Sato, R., Kuroiwa, S., Nakanishi, T., Nishimura, S. –I., Osaka, T. Anal. Chem. 85, 5641-5644 (2013).
   PMID: 23675869
9. “Site-specific conformational alteration induced by sialylation of MUC1 tandem repeating glycopeptides at an epitope region for the anti-KL-6 monoclonal antibody.”
   Matsushita, T., Ohyabu, N., Fujitani, N., Naruchi, K., Shimizu, H., Hinou, H., Nishimura, S. –I. Biochemistry 52, 402-414 (2013).
   PMID: 23259747
10. “A novel approach for the parallel synthesis of glycopeptides by combining solid-phase peptide synthesis and dendrimer-supported enzymatic modifications.”
    Matsushita, T., Handa, S., Naruchi, K., Garcia-Martin, F., Hinou, H., Nishimura, S. –I. Polym. J. 45, 854-862 (2013).

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学会発表

1. “Preparation of glycoamino acid building blocks for microwave-assisted solid phase synthesis of bioactive glycopeptides.”Naruchi, K., Tan, S. R., Ravi Kumar, H. V., Miyoshi, R., Hinou, H., Nishimura, S. –I.第52回ペプチド討論会、平塚市中央公民館、2015年11月16日
2. “Epitope-defined antibodies as new class therapeutic and diagnostic reagents: Toward personalized medicine from chemical glycobiology.”Nishimura, S. –I. 23rd International Symposium on Glycoconjugates, Split, Croatia, September 15-20, 2015  Keynote lecture
3. “効率的な複合糖質合成技術を基盤としたエピトープ探索”成地健太郎、GFRG研究会（依頼講演）、天王洲アイル、2013年8月28日
4. “Glycomics and drug discovery”Nishimura, S. –I. 26th International Carbohydrate Symposium, Madrid, Spain, July 22-27, 2013  Invited lecture