Antibody Purification and Labeling

Antibody sequencing

We provide sequencing service of valiable region of mouse monoclonal antibody. The antibody sequence information is essential for antibody engineering including humanization and drug development.
We analyze the sequence not only for hybridoma we have established as service, but also for hybridomas you already have.

	Service contents	Deriverables	Standard time period	Price
Antibody sequencing	・Preparation of total RNA from mouse hybridoma. ・Synthesis of cDNA ・PCR amplification of variable region with primer mix specific for heavy chain and light chain each Sequencing of PCR amplified fragments ・Isolation of leader sequence by 5′ RACE PCR ・Identification of sequences from leader sequence to variable region ※Sample condition to be provided Hybridoma (mouse) cell pellet (1 x 106 cells or more) Antibody isotype should be IgG, and light chain should be κ chain.	Sequence data from leader to variable region in heavy chain and light chain	2 months	JPY 500,000/ clone
Options	Culture of hybridoma	–	–	please inquire
Isotyping of antibody	–	–	JPY 90,000/ antibody

Service contents

Deriverables

Standard time period

Price

Antibody sequencing

・Preparation of total RNA from mouse hybridoma.
・Synthesis of cDNA
・PCR amplification of variable region with primer mix specific
for heavy chain and light chain each Sequencing of PCR amplified fragments
・Isolation of leader sequence by 5′ RACE PCR
・Identification of sequences from leader sequence to variable region

※Sample condition to be provided
Hybridoma (mouse) cell pellet (1 x 106 cells or more)
Antibody isotype should be IgG, and light chain should be κ chain.

Sequence data from leader
to variable region
in heavy chain and
light chain

2 months

JPY 500,000/
clone

Options

Culture of hybridoma

–

please inquire

Isotyping of antibody

–

JPY 90,000/
antibody

	Content of work	Quantity	Price
Purification of Polyclonal Antibodies	・Affinity purification from anti-sera. ・ELISA (titer) analysis.	20mL~	JPY120,000~
Purification of Monoclonal Antibodies	・Hybridoma culture ・Purification by ProteinG column chromatography ・ELISA (titer) analysis	~100mL	JPY200,000~
Antibody labeling	Biotin	~500μg	JPY70,000
	Horseradish peroxidase (HRP)	~500μg	JPY80,000
	Alkaline phosphatase (ALP)	~500μg	JPY100,000
	Fluorescein isothiocyanate (FITC)	~500μg	JPY100,000

Large scale preparation of monoclonal antibodies

	Contents	Scale of culture	Period	Price
High density cell culture	High density culture of hybridoma in serum free medium (SFM) Collection of cultured supernatant	1L	1.5 months	JPY250,000
High density cell culture		5L	2 months	JPY550,000
High density cell culture and IgG purification	High density culture of hybridoma in serum free medium (SFM) Collection of cultured supernatant Purification by ProteinG column chromatography Measurement of protein concentration	1L	2.5 months	JPY550,000
High density cell culture and IgG purification		5L	3.5 months	JPY1,200,000

※Please inquire about other custom services and large scale services.