Antibody Products

Product NameAnti Human Macrophage Surface Antigen Monoclonal Antibody ( Clone No. AM-3K )

Cat. No.：
KT013
KT013

Volume： 50μg/200μL
Price： inquiry
Data Sheet Data Sheet
Antigen： human alveolar macrophage
GenBank ID： –
Immunizing animal： mouse
Purification： ProteinG
Antibody subclass： IgG1
Application Immuno HistoChemistry(10μg/mL)
Cross React Human
Others This anti-macrophage monoclonal antibody, AM-3K, was produced by using human alveolar macrophages as immunogen. AM-3K reacts intensely with most of macrophages in lymphoreticular organs and in many other organs and tissues. AM-3K also reacts with the macrophages in many pathological conditions. However, this antibody does not react with dendritic cell population, such as epidermal Langerhans cells, interdigitating cells in the paracortex of lymph nodes, nor follicular dendritic cells. Lymphocytes, granulocytes and freshly isolated blood monocytes are also negative. Reaction products for AM-3K were found on the cytoplasmic membrane of macrophages by immunoelectron microscopy.
In both cryostat sections and formalin-fixed paraffin sections, this antibody recognizes the antigen presenting on the cell surface membrane of tissue macrophages, but not monocytes or dendritic cells.
The molecular weights of the antigen recognized by AM-3K are 120 and 70 kDa.
Reference
  • J Pathol. 1996 Feb;178(2):207-14.
    AM-3K, a novel monoclonal antibody specific for tissue macrophages and its application to pathological investigation.
    Zeng L, Takeya M, Takahashi K.

  • Vet Pathol. 2000 Mar;37(2):168-76.
    Distribution of cells immunopositive for AM-3K, a novel monoclonal antibody recognizing human macrophages, in normal and diseased tissues of dogs, cats, horses, cattle, pigs, and rabbits.
    Yamate J, Yoshida H, Tsukamoto Y, Ide M, Kuwamura M, Ohashi F, Miyamoto T, Kotani T, Sakuma S, Takeya M.

  • Apoptosis. 2001 Aug;6(4):279-90.
    Progressively developed myocardial apoptotic cell death during late phase of reperfusion.
    Zhao ZQ, Velez DA, Wang NP, Hewan-Lowe KO, Nakamura M, Guyton RA, Vinten-Johansen J.

  • Circulation. 2002 Jul 16;106(3):362-7.
    Probucol attenuates left ventricular dysfunction and remodeling in tachycardia-induced heart failure: roles of oxidative stress and inflammation.
    Nakamura R, Egashira K, Machida Y, Hayashidani S, Takeya M, Utsumi H, Tsutsui H, Takeshita A.

  • Arterioscler Thromb Vasc Biol. 2004 Jan;24(1):181-6.
    Long-term inhibition of Rho-kinase suppresses neointimal formation after stent implantation in porcine coronary arteries: involvement of multiple mechanisms.
    Matsumoto Y, Uwatoku T, Oi K, Abe K, Hattori T, Morishige K, Eto Y, Fukumoto Y, Nakamura K, Shibata Y, Matsuda T, Takeshita A, Shimokawa H.

  • Pathol Int. 2009 May;59(5):300-5.
    Detection of M2 macrophages and colony-stimulating factor 1 expression in serous and mucinous ovarian epithelial tumors
    Kawamura K, Komohara Y, Takaishi K, Katabuchi H, Takeya M.

  • Pathol Int. 2010 Jan;60(1):27-34.
    Macrophages in Langerhans cell histiocytosis are differentiated toward M2 phenotype: their possible involvement in pathological processes.
    Ohnishi K, Komohara Y, Sakashita N, Iyama K, Murayama T, Takeya M.

  • Cancer Sci. 2010 Oct;101(10):2128-36.
    Involvement of M2-polarized macrophages in the ascites from advanced epithelial ovarian carcinoma in tumor progression via Stat3 activation.
    Takaishi K, Komohara Y, Tashiro H, Ohtake H, Nakagawa T, Katabuchi H, Takeya M.

  • Cancer Sci 2011 102: 206-211,
    Corosolic acid inhibits glioblastoma cell proliferation by suppressing the activation of signal transducer and activator of transcription-3 and nuclear factor-kappa B in tumor cells and tumor-associated macrophages.
    Fujiwara Y, Komohara Y, Ikeda T, Takeya M.
Collaborators Dr. Takeya M., Second Department of Pathology, Kumamoto University School of Medicine, Kumamoto, Japan

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How to Order

Please contact one of following international distributors.

Cosmo Bio USA, Inc.
E-mail : info@cosmobiousa.com
TEL : +1-760-431-4600
FAX : +1-760-431-4600
Funakoshi Corporation
E-mail : reagent@funakoshi.co.jp
TEL : +81-3-5684-1620
FAX : +81-3-5684-1775
FUJIFILM Wako Pure Chemical Corporation
E-mail : ffwk-labchem-tec@fujifilm.com
TEL : 0120-052-099 (only in Japan)
FAX : 0120-052-806 (only in Japan)
Sceti K.K.
E-mail : export@sceti.co.jp
TEL : +81-3-5510-2652
FAX : +81-3-5510-0133