Antibody Products

Product NameAnti Human PEDF Monoclonal Antibody ( Clone No.14K )

Cat. No.：
KM037
KM037

Volume： 50μg/200μL
Price： inquiry
Data Sheet Data Sheet
Antigen： partial peptide
GenBank ID： 5176
Immunizing animal： mouse
Purification： ProteinG
Antibody subclass： IgG1
Application Western Blotting(0.5-1.0μg/mL)
Cross React Human
Others Pigment Epithelium-Derived Factor ( PEDF ), a protein previously shown to have neurotrophic activity, is indispensable for neuronal development, differentiation and existence adjacent to retina.
The amount of inhibitory PEDF produced by retinal cells was positively correlated with oxygen concentrations,suggesting that its loss play a permissive role in ischemia-driven retinal neovascularization. So that, PEDF is likely to contribute to regulate of blood vessels growth in eye by creating a permissive environment for angiogenesis when oxygen is limiting and an inhibitory environment oxygen concentrations are normal or high ( Science Vol 285: 245-248,1999).
PEDF may be a therapeutic target pathologic ocular neovascularization as well as for retinoblastomas.
This antibody could detect PEDF with western-blotting method in medium of retinal pigment epithelium cell. This antibody is useful in research for angionenesis and neuronal system.
Reference
  • Biochem Biophys Res Commun. 2002 Aug 30;296(4):877-82.
    Pigment epithelium-derived factor protects cultured retinal pericytes from advanced glycation end product-induced injury through its antioxidative properties.
    Yamagishi S, Inagaki Y, Amano S, Okamoto T, Takeuchi M, Makita Z.

  • Cell Tissue Res. 2005 Jun;320(3):437-45.
    Pigment epithelium-derived factor (PEDF) blocks angiotensin II signaling in endothelial cells via suppression of NADPH oxidase: a novel anti-oxidative mechanism of PEDF.
    Yamagishi S, Nakamura K, Ueda S, Kato S, Imaizumi T.

  • J Clin Endocrinol Metab. 2006 Jun;91(6):2447-50.
    Elevated serum levels of pigment epithelium-derived factor in the metabolic syndrome.
    Yamagishi S, Adachi H, Abe A, Yashiro T, Enomoto M, Furuki K, Hino A, Jinnouchi Y, Takenaka K, Matsui T, Nakamura K, Imaizumi T.

  • Microvasc Res. 2006 May;71(3):222-6.
    Pigment-epithelium-derived factor (PEDF) inhibits angiotensin-II-induced vascular endothelial growth factor (VEGF) expression in MOLT-3 T cells through anti-oxidative properties.
    Yamagishi S, Matsui T, Nakamura K, Yoshida T, Shimizu K, Takegami Y, Shimizu T, Inoue H, Imaizumi T.

  • FEBS Lett. 2006 May 15;580(11):2788-96.
    Pigment epithelium-derived factor (PEDF) inhibits advanced glycation end product (AGE)-induced C-reactive protein expression in hepatoma cells by suppressing Rac-1 activation.
    Yoshida T, Yamagishi S, Nakamura K, Matsui T, Imaizumi T, Takeuchi M, Ueno T, Sata M.

  • J Biol Chem. 2006 Jul 21;281(29):20213-20.
    Pigment epithelium-derived factor inhibits advanced glycation end product-induced retinal vascular hyperpermeability by blocking reactive oxygen species-mediated vascular endothelial growth factor expression.
    Yamagishi S, Nakamura K, Matsui T, Inagaki Y, Takenaka K, Jinnouchi Y, Yoshida Y, Matsuura T, Narama I, Motomiya Y, Takeuchi M, Inoue H, Yoshimura A, Bucala R, Imaizumi T.

  • Microvasc Res. 2006 Jul-Sep;72(1-2):86-90.
    Pigment epithelium-derived factor (PEDF) prevents diabetes- or advanced glycation end products (AGE)-elicited retinal leukostasis.
    Yamagishi S, Matsui T, Nakamura K, Takeuchi M, Imaizumi T.

  • Life Sci. 2006 Oct 19;79(21):1981-7.
    Pigment epithelium-derived factor (PEDF) blocks the interleukin-6 signaling to C-reactive protein expression in Hep3B cells by suppressing Rac-1 activation.
    Yoshida T, Yamagishi S, Nakamura K, Matsui T, Imaizumi T, Inoue H, Ueno T, Sata M.

  • Horm Metab Res. 2006 Aug;38(8):546-8.
    Pigment epithelium-derived factor (PEDF) blocks angiotensin II-induced T cell adhesion to endothelial cells by suppressing intercellular adhesion molecule-1.
    Yamagishi SI, Kikuchi S, Nakamura K, Matsui T, Makino T, Norisugi O, Shimizu T, Inoue H, Imaizumi T.

  • Med Chem. 2006 May;2(3):265-9.
    Pigment epithelium-derived factor (PEDF) blocks angiotensin II-induced T cell proliferation by suppressing autocrine production of interleukin-2.
    Yamagishi S, Kikuchi S, Nakamura K, Matsui T, Takeuchi M, Inoue H.

  • Ophthalmic Res. 2007;39(2):92-7.
    Pigment-epithelium-derived factor suppresses expression of receptor for advanced glycation end products in the eye of diabetic rats.
    Yamagishi S, Matsui T, Nakamura K, Yoshida T, Takeuchi M, Inoue H, Yoshida Y, Imaizumi T.

  • Horm Metab Res. 2007 Mar;39(3):233-5.
    Pigment epithelium-derived factor (PEDF) blocks advanced glycation end product (AGE)-induced angiogenesis in vitro.
    Yamagishi SI, Nakamura K, Matsui T, Yoshida T, Takeuchi M, Imaizumi T.

  • Am J Pathol. 2007 Jun;170(6):2159-70.
    Pigment epithelium-derived factor inhibits neointimal hyperplasia after vascular injury by blocking NADPH oxidase-mediated reactive oxygen species generation.
    Nakamura K, Yamagishi S, Matsui T, Yoshida T, Takenaka K, Jinnouchi Y, Yoshida Y, Ueda S, Adachi H, Imaizumi T.

  • Protein Pept Lett. 2007;14(6):615-7.
    Pigment epithelium-derived factor (PEDF) inhibits angiotensin II-induced smooth muscle cell proliferation through its anti-oxidative properties.
    Yamagishi S, Matsui T, Nakamura K, Imaizumi T.

  • Protein Pept Lett. 2007;14(8):832-5.
    Pigment epithelium-derived factor (PEDF) prevents advanced glycation end products (AGEs)-elicited endothelial nitric oxide synthase (eNOS) reduction through its anti-oxidative properties.
    Yamagishi S, Ueda S, Matsui T, Nakamura K, Imaizumi T, Takeuchi M, Okuda S.

  • J Int Med Res. 2007 Nov-Dec;35(6):896-9.
    Pigment epithelium-derived factor inhibits vascular endothelial growth factor-induced vascular hyperpermeability both in vitro and in vivo.
    Yamagishi S, Abe R, Jinnouchi Y, Matsui T, Imaizumi T, Inoue H.

  • Horm Metab Res. 2008 Sep;40(9):620-5.
    Pigment epithelium-derived factor (PEDF) ameliorates advanced glycation end product (AGE)-induced hepatic insulin resistance in vitro by suppressing Rac-1 activation.
    Yoshida T, Yamagishi S, Nakamura K, Matsui T, Imaizumi T, Takeuchi M, Koga H, Ueno T, Sata M.

  • Clin Exp Med. 2009 Mar;9(1):73-6.
    Administration of pigment epithelium-derived factor prolongs bleeding time by suppressing plasminogen activator inhibitor-1 activity and platelet aggregation in rats.
    Yamagishi S, Matsui T, Nakamura K, Takenaka K.

  • Nephrol Dial Transplant. 2009 May;24(5):1397-406.
    Administration of pigment epithelium-derived factor (PEDF) reduces proteinuria by suppressing decreased nephrin and increased VEGF expression in the glomeruli of adriamycin-injected rats.
    Fujimura T, Yamagishi S, Ueda S, Fukami K, Shibata R, Matsumoto Y, Kaida Y, Hayashida A, Koike K, Matsui T, Nakamura K, Okuda S.

  • Diabetes Metab Res Rev. 2009 Mar;25(3):266-71.
    Pigment epithelium-derived factor (PEDF) prevents platelet activation and aggregation in diabetic rats by blocking deleterious effects of advanced glycation end products (AGEs).
    Yamagishi S, Matsui T, Takenaka K, Nakamura K, Takeuchi M, Inoue H.

  • Diabetes Metab Res Rev. 2009 Oct;25(7):678-86.
    Protective role of pigment epithelium-derived factor (PEDF) in early phase of experimental diabetic retinopathy.
    Yoshida Y, Yamagishi S, Matsui T, Jinnouchi Y, Fukami K, Imaizumi T, Yamakawa R.

  • Int J Cardiol. 2010 Oct 8;144(2):283-5.
    Pigment epithelium-derived factor (PEDF) inhibits diabetes- or advanced glycation end product (AGE)-induced platelet CD40 ligand overexpression in rats.
    Yamagishi S, Matsui T, Ueda S, Takeuchi M.

  • Am J Pathol. 2010 Jan;176(1):168-76.
    Pigment epithelium-derived factor inhibits lysosomal degradation of Bcl-xL and apoptosis in HepG2 cells.
    Kawaguchi T, Yamagishi S, Itou M, Okuda K, Sumie S, Kuromatsu R, Sakata M, Abe M, Taniguchi E, Koga H, Harada M, Ueno T, Sata M.

  • J Dermatol Sci. 2010 Mar;57(3):183-91.
    Topical application of anti-angiogenic peptides based on pigment epithelium-derived factor can improve psoriasis.
    Abe R, Yamagishi S, Fujita Y, Hoshina D, Sasaki M, Nakamura K, Matsui T, Shimizu T, Bucala R, Shimizu H.

  • Microvasc Res. 2010 Sep;80(2):227-32.
    Pigment epithelium-derived factor inhibits advanced glycation end product-elicited mesangial cell damage by blocking NF-kappaB activation.
    Ide Y, Matsui T, Ishibashi Y, Takeuchi M, Yamagishi S.
Collaborators Dr. I. Morita and Dr. K. Ohno Tokyo medical and dental university

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