Antibody Products

Product NameAnti Pentosidine Monoclonal Antibody ( Clone No. PEN-12 ) Biotin conjugated

Cat. No.：
KH012-01
sample

Volume： 50μg/200μL
Price： inquiry
Data Sheet Data Sheet
Antigen： Pentosidine-HSA
GenBank ID： –
Immunizing animal： mouse
Purification： ProteinG
Antibody subclass： IgG1
Application ELISA(0.1-1.0μg/mL) Immuno HistoChemistry(5.0-10.0μg/mL)
Cross React Human Mouse Rat Other
Others Reaction of protein amino groups with glucose leads, through the early products such as a Schiff base and Amadori rearrangement products, to the formation of advanced glycation end products (AGEs). Recent immunological studies using anti-AGEs antibody (6D12) demonstrated the presence of AGEs-modified proteins in several human tissues: (ⅰ) human lens (nondiabetic and noncataractous), (ⅱ) renal proximal tubules in patients with diabetic nephropathy and chronic renal failure, (ⅲ) diabetic retina, (ⅳ) peripheral nerves of diabetic neuropathy, (ⅴ) atherosclerotic lesions of arterial walls, (ⅵ)β2-microglobulin forming amyloid fibrils in patients with hemodialysis-related amyloidosis, (ⅶ) senile plaques of patients with Alzheimer’s disease, (ⅷ) the peritoneum of CAPD patients, (ⅸ) skin elastin in actinic elastosis, and (ⅹ) ceriod/lipofuscin deposits. These results suggest a potential role of AGEs-modification in normal aging as well as age-enhanced disease processes. This antibody named as 6D12 has been used to demonstrate AGEs-modified proteins in these human tissues, indicating potential usefulness of this antibody for histochemical identification and biochemical quantification of AGEs-modified proteins.
Pentosidine is one of the Maillard compounds identified by Monnier et al in 1989. It has been proved to cross-link Arginine to Lysine residue and be detected in β 2-microglobulin from patients with hemodialysis- related amyloidosis.
Reference
  • Ann N Y Acad Sci. 2008 Apr;1126:328-32.
    Modification of vimentin: a general mechanism of nonenzymatic glycation in human skin.
    Kueper T, Grune T, Muhr GM, Lenz H, Wittern KP, Wenck H, Stab F, Blatt T.

  • Toxicology. 2010 Jan 31;268(1-2):89-97.
    Involvement of advanced glycation end-products, pentosidine and N(epsilon)-(carboxymethyl)lysine, in doxorubicin-induced cardiomyopathy in rats.
    Moriyama T, Kemi M, Okumura C, Yoshihara K, Horie T.

  • J Immunol. 2011 Mar 1;186(5):3248-57.
    Septic shock is associated with receptor for advanced glycation end products ligation of LPS.
    Yamamoto Y, Harashima A, Saito H, Tsuneyama K, Munesue S, Motoyoshi S, Han D, Watanabe T, Asano M, Takasawa S, Okamoto H, Shimura S, Karasawa T, Yonekura H, Yamamoto H.
Collaborators —

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How to Order

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Cosmo Bio USA, Inc.
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Funakoshi Corporation
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FUJIFILM Wako Pure Chemical Corporation
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Sceti K.K.
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FAX : +81-3-5510-0133